Clone ID: Y135
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1520-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 85-95 kDa
UniProt ID: P47736
Alternative names: Rap1GAP
Description: Rap1GAP, an 85/95kDa protein, is a member of a family of GAPs specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5). Specificity: A synthetic peptide corresponding to residues in the C-terminus of human Rap1GAP was used as an immunogen.
Recommended Dilutions:
WB: 1:5,000-10,000
IHC: 1:100-250
ICC: 1:100-250
Flow Cytometry: 1:40
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Jurkat cell lysate using anti-Rap1GAP (C-term) RabMAb (catalog #1520-1), dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-Rap1GAP (C-term) RabMAb (catalog #1520-1). |
Description References:
| 1. |
Rubinfeld, B. et al. Cell 65, 1033−1042 (1991) |
| 2. |
Polakis, P. G., Rubinfeld, B., Evans, T., and McCormick, F. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 239-243 |
| 3. |
Nice, E. C., Fabri, L., Hammacher, A., Holden, J., Simpson, R. J., and Burgess, A. W. (1992) J. Biol. Chem. 267, 1546-1553 |
| 4. |
Daumke, O. et al. Nature, Vol. 429, May 2004, 197-201 |
| 5. |
Manning, B.D. et al. Trends. Biochem. Sci. 28, 573-576 (2003) |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
