Clone ID: Y123
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1515-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human
Mol. Weight: 56 kDa
UniProt ID: P06239
Alternative names: p56-LCK, Lymphocyte cell-specific protein-tyrosine kinase, LSK, T cell-specific protein-tyrosine kinase
Description: The human T-cell or lymphocyte specific gene Lck/p56 is a member of the Src family of non-receptors tyrosine kinase. Mostly expressed in T cells, Lck has been shown to be critical for the normal development of T lymphocytes (1). Additionally, the N-terminal region of the tyrosine kinase Lck interacts with the cytoplasmic domains of CD4 and CD8 (2). Autophosphorylation at Tyr-394 appears to be required for maximum catalytic activity (3) but it can also be suppressed by phosphorylation of the carboxyl-terminal tyrosine Tyr-505 (4). Specificity: A synthetic peptide corresponding to residues before SH3 domain of human Lck was used as immunogen. The antibody does not cross-react with other SRC family member.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:250-500
ICC: 1:100
Flow Cytometry: 1:20
IP: 1:200
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on Jurkat cell lysate using anti-Lck/p56 RabMAb (catalog #1515-1), dilution 1:1,000. |
B. Immunohistochemical analysis of paraffin-embedded human lymphoma using anti-Lck/p56 RabMAb (catalog #1515-1). |
Description References:
| 1. |
Rudd, C. E., Trevillyan, J. M., Dasgupta, J. D., Wong, L. L., and Schlossman, S. F. (1988) Proc. Natl. Acad. Sci., U. S. A. 85, 5190 |
| 2. |
Veillette, A., M. A. Bookman, E. M. Horak, J. B. Bolen. 1988. The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56 lck. Cell 55: 301 |
| 3. |
Boulet, I., Fagard, R., and Fischer, S. (1987) Bichem. Biophys. Res. Commun. 149, 56 |
| 4. |
Nakamura, K., Hori, T., Sato, N., Sugie, K., Kawakami, T., and Yodoi, J. (1993) Oncogene 8, 3133-3139 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
