Clone ID: Y125
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1514-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 77 kDa
UniProt ID: P05771-2
Alternative names:
Description: PKCβII is a member of the serine/threonine protein kinase C (PKC) family (1) that includes 10 closely related isozymes classified as the conventional, the novel and the atypical. The conventional PKCβII is implicated in diverse cellular process including secretion, proliferation, differentiation, apoptosis, permeability, migration & hypertrophy (2-3). PKCβII structure consists of a C-terminal catalytic region, containing an activator-binding domain and a Ca2+-binding domain, and a regulatory domain. The catalytic domain is activated by undergoing multiple serine/threonine and tyrosine phosphorylation that are either autocatalytic, or catalyzed by PKC kinases (4). PKCβII and PKCβI differ only in their C-terminal (5). Specificity: A synthetic peptide corresponding to residues near C-terminus of human PKC beta-II was used as immunogen. The antibody does not cross-react with human PKC beta-I. Predicted to cross-react with bovine PKC beta, and pig PKC beta-II, based on sequence homology
Recommended Dilutions:
WB: 1:500
IHC: 1:100-250
ICC: 1:250-500
Flow Cytometry: 1:50
IP: 1:60
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on K562 cell lysate using anti-PKCβII RabMAb (catalog #1514-1), dilution 1:500 |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti- PKCβII RabMAb (catalog #1514-1). |
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| C. Immunofliorescent staining of HeLa cells using anti-PKCβII RabMAb (catalog #1514-1). |
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Description References:
| 1. |
Coussens L, Parker PJ, Rhee L, Yang-Feng TL, Chen E, Waterfield MD, Francke U, Ullrich A. (986) Science. Aug 22;233(4766):859-66. |
| 2. |
Mellor, H., and Parker, P. J. (1998) Biochem. J. 332, 281-292 |
| 3. |
Nishizuka, Y. (1995) FASEB J. 9, 484-496 |
| 4. |
Parekh, D. B., Ziegler, W., and Parker, P. J. (2000) EMBO J. 19, 496-503 |
| 5. |
YS Niino, S Ohno and K Suzuki. (1992) J. Biol. Chem., Vol. 267, Issue 9, 6158-6163, 03 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
