Clone ID: Y119
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1512-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 36 kDa
UniProt ID: P67775
Alternative names: PP2A-alpha, Replication protein C, RP-C
Description: Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways (1-3). PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A (4). Specificity: A synthetic peptide corresponding to residues near C-terminus of human PP2A was used as immunogen. This antibody detects both alpha and beta isoforms of PP2A.
Recommended Dilutions:
WB: 1:5,000-10,000
IHC: 1:250-500
ICC: 1:50
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on T47-D cell lysate using anti-PP2A RabMAb (catalog #1512-1), dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PP2A RabMAb (catalog #1512-1). |
Product References:
| 1. | Singh AP, Bafna S, Chaudhary K, et al, Genome-wide expression profiling reveals transcriptomic variation and perturbed gene networks in androgen-dependent and androgen-independent prostate cancer cells ,Cancer Letters 259 (2008) 28?38
[Application: WB, IHC] |
Description References:
| 1. |
Janssens, V. and J. Goris. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J. 353: 417 |
| 2. |
Zabrocki, P., et al. Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol. Microbiol. 43: 835 |
| 3. |
Milward, T.A., et al. Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem. Sci. 24: 186 |
| 4. |
Chen, J., et al. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science 257: 1261 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
