Clone ID: Y117
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1511-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 68 kDa
UniProt ID: P19525
Alternative names: Interferon-inducible RNA-dependent protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, Protein kinase RNA-activated, PKR, p68 kinase, P1/eIF-2A protein kinase
Description: Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the α-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2α), inhibiting protein synthesis (1,2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3). Specificity: A synthetic peptide corresponding to residues near C-terminus of human PKR was used as immunogen. The antibody does not cross-react with other GCN2 family members.
Recommended Dilutions:
WB: 1:5,000-10,000
IHC: 1:100
ICC: 1:250-500
IP: 1:80
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on MCF-7 cell lysate using anti-PKR (C-term) RabMAb (catalog # 1511-1), 1:10,000 dilution. |
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-PKR (C-term) RabMAb (catalog #1511-1). |
|
|
| C. Immunofluorescent staining of HeLa cells using anti-PKR (C-term) RabMAb (catalog #1511-1). |
|
Description References:
| 1. |
Williams, B.R. PKR; a sentinel kinase for cellular stress. Oncogene 18: 6112 |
| 2. |
Cuddihy, A.R., et al. The double-stranded RNA activated protein kinase PKR physically associates with the tumor suppressor p53 protein and phosphorylates human p53 on serine 392 in vitro. Oncogene 18:2690-2702 (1999). |
| 3. |
Zhang, F., et al. Binding of double-stranded RNA to protein kinase PKR is required for dimerization and promotes critical autophosphorylation events in the activation loop. J. Biol. Chem. 276: 24946 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
