Clone ID: Y124
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1510-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 80 kDa
UniProt ID: P17252
Alternative names: PKC-alpha, PKC-A
Description: Activation of PKC plays a crucial role in tranducing signals for a variety of cellular responses, including tumor proliferation (1). PKC isoforms have been classified into three groups: classical, novel, and atypical. Members of these three PKC groups contain an autoinhibitory domain that binds to the substrate binding site in the catalytic domain, preventing its activation in the absence of cofactors or activators (2,3). The activity of PKC is under the control of three distinct phosphorylation events, including that of the Thr641 autophosphorylation site (4). Specificity: A synthetic peptide corresponding to residues in C-terminus of human PKCa was used as immunogen.
Recommended Dilutions:
WB: 1:5,000-10,000
IHC: 1:250-500
ICC: 1:250-500
Flow Cytometry: 1:100
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on 293 cell lysate using anti- PKCα (C-term) RabMAb (cat. # 1510-1), 1:10,000 dilution. |
B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-PKCα (C-term) RabMAb (catalog #1510-1). |
|
|
| C. Immunofluorescent staining of HeLa cells using anti- PKCα (C-term) RabMAb (catalog #1510-1). |
|
Description References:
| 1. |
J Nishizuka, Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature 308: 693 |
| 2. |
Newton, A.C. Protein kinase C: structure, function, and regulation. J. Biol. Chem. 270: 28495 |
| 3. |
Mellor, H. and P. Perker. The extended protein kinase C superfamily. Biochem. J. 332: 281 |
| 4. |
Keranen, L., et al. Protein kinase C is regulated in vivo by three functionally distinct phosphorylations. Curr. Biol. 5: 1394 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
