Clone ID: YE353
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1509-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human,Mouse, Rat
Mol. Weight: 116 kDa
UniProt ID: Q14289
Alternative names: Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Cell adhesion kinase beta, CAK beta, Calcium-dependent tyrosine kinase, CADTK, Related adhesion focal tyrosine kinase, RAFTK
Description: A Proline rich protein tyrosine kinase (Pyk2) is a focal adhesion kinase (FAK) related protein kinase protein also known as cell adhesion kinase (CAK), related adhesion focal tyrosine kinase (RAFTK), or calcium-dependent PTK (CADTK) (3.4). Highly expressed in the nervous system, protein tyrosine kinase (Pyk2) is phosphorylated on the tyrosine residue by a variety of G-protein-coupled receptors & extracellular signals that increase the level of Ca + intracellulary (1,2) Specificity: A synthetic peptide corresponding to residues in the N-terminus of human PYK2 was used as immunogen. The antibody does not cross-react with other FAK family members.
Recommended Dilutions:
WB: 1:500
IHC: 1:250-500
ICC: 1:100
IP: 1:80
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on Jurkat cell lysate using anti-Pyk2 (N-term) RabMAb (catalog #1509-1), dilution 1:5,000 |
B. Immunohistochemical analysis of paraffin-embedded human normal brain tissue using anti-Pyk2 (N-term) RabMAb (catalog #1509-1). |
|
|
| C. Immunofluorescent staining of PC12 cells using anti-Pyk2 (N-term) RabMAb (catalog #1509-1). |
|
Description References:
| 1. |
Andreev J., Simon J.-P., Sabatini D.D., Kam J., Plowman G., Randazzo P.A., Schlessinger J.; "Identification of a new Pyk2 target protein with Arf-GAP activity."; Mol. Cell. Biol. 19:2338-2350(1999). |
| 2. |
Schlessinger J., 1995. Protein tyrosine kinase PYK2 involved in calcium-induced regulation of ion channel and MAP kinase functions. Nature376:737-745 |
| 3. |
Candice K. Klingbeil, Christof R. Hauck, et al. Targeting Pyk2 to |
| 4. |
Avraham H., Park S.Y., Schinkmann K., Avraham S., 2000. RAFTK/Pyk2-mediated cellular signalling. Cell. Signal12:123-133 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
