Clone ID: Y112
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1507-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse
Mol. Weight: 95 kDa
UniProt ID: O60934
Alternative names: Nijmegen breakage syndrome protein 1, Cell cycle regulatory protein p95
Description: Nibrin (also known as NBS1 or p95) contains 2 modules found in cell cycle check point; a forkhead-associated domain & a BRCA1 C-teminal repeat (1). In mammalian cells, Nibrin forms a complex with Mre11 & Rad50 (MRN). Nuclear localization of the MRN complex is relevant for chromosomal stability, meiotic recombination, DNA repair & telomere maintenance in eukaryotic cells(2-4). Mutation in Nibrin & Mre11 causes respectively two related disorders, Nijmegen breakage syndrome (NBS) & Ataxia-Telangiectasia- like disorder (ATLD). Specificity: A synthetic peptide corresponding to residues near C-terminus of human Nibrin was used as immunogen
Recommended Dilutions:
WB: 1:500
IHC: 1:50
ICC: 1:50
IP: 1:60
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on analysis on Hela cell lysate using anti-Nibrin/NBS1 RabMAb (catalog #1507-1), dilution 1:500. |
B. Immunohistochemical analysis of paraffin-embedded human skin carcinoma using anti-Nibrin/NBS1 RabMAb (catalog #1507-1). |
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| C. Immunofluorescent staining of HeLa cells using anti-Nibrin/NBS1 RabMAb (catalog #1507-1). |
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Description References:
| 1. |
Trujillo, K. M., Yuan, S. S., Lee, E. Y., and Sung, P. (1998) J. Biol. Chem. 273, 21447-21450 |
| 2. |
Dolganov, G. M., Maser, R. S., Novikov, A., Tosto, L., Chong, S., Bressan, D. A., and Petrini, J. H. (1996) Mol. Cell. Biol. 16, 4832-4841 |
| 3. |
Zhu, J., Petersen, S., Tessarollo, L. & Nussenzweig, A. (2001) Curr. Biol. 11, 105-109 |
| 4. |
Varon, R., Vissinga, C., Platzer, M., Cerosaletti, K. M., Chrzanowska, K. H., Saar, K., Beckmann, G., Seemanova, E., Cooper, P. R., Nowak, N. J., et al. (1998) Cell 93, 467-476. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
