Clone ID: E272
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1501-1
Size: 100ul
Price: $ 250
Recommended Applications: IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 23 kDa
UniProt ID: P07766
Alternative names: T-cell surface antigen T3/Leu-4 epsilon chain
Description: CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk (3). Specificity: A synthetic peptide corresponding to residues in C-terminal cytoplasmic domain of human CD-3 epsilon chain was used as immunogen.
Recommended Dilutions:
IHC: 1:100
ICC: 1:100-250
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis of immunoprecipitation pellets from Jurkat cell lysate or 1XPBS (negative control) using anti-CD3 RabMAb (catalog # 1501-1 ), 1:50 dilution. |
B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti-CD3 RabMAb(cat. #1501-1). |
References:
| 1. |
Weiss, A., et al. Signal transduction by the T cell antigen receptor. Sem. Immunol. 3: 313 |
| 2. |
Siegel, J.N., et al. Multiple signal transduction pathways activated through the T cell receptor for antigen. Semin Immunol. 3: 325 |
| 3. |
Futterer, K., et al. Structural basis for Syk tyrosine kinase ubiquity in signal transduction pathways revealed by the crystal structure of its regulatory SH2 domains bound to a dually phosphorylated ITAM peptide. J Mol Biol. 281: 523 |
Orders:
877-772-2622 (US and Canada)
650-443-2276 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
