Clone ID: E306
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1496-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse
Mol. Weight: 40 kDa
UniProt ID: P16220
Alternative names: CREB
Description: CREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3). Specificity: A synthetic peptide corresponding to residues before DNA binding motif of human CREB was used as immunogen. This antibody can also recognize the splice isoform of CREB. Predicted to cross-react with bovine, based on sequence homology.
Recommended Dilutions:
WB: 1:500-1,000
IHC: 1:250-500
ICC: 1:250-500
Flow Cytometry: 1:40
IP: 1:80
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on A431 cell lysate using anti-CREB RabMAb (catalog #1496-1), dilution 1:1,000. |
B. Immunohistochemical analysis of paraffin-embedded prostate carcinoma using anti-CREB RabMAb (catalog #1496-1). |
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| C. Immunofluorescent staining of PC-12 cells using anti-CREB RabMAb (catalog #1496-1). |
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Product References:
| 1. | Keller MJ et. al., Reversal of Human Cytomegalovirus Major Immediate-Early Enhancer/Promoter Silencing in Quiescently Infected Cells via the Cyclic-AMP Signaling Pathway ,J. Virol., Feb 2007
[Application: WB] |
Description References:
| 1. |
Sheng, M., et al. CREB: a Ca(2+)-regulated transcription factor phosphorylated by calmodulin-dependent kinases. Science 252: 1427 |
| 2. |
Xing, J., et al. Coupling of the RAS-MAPK pathway to gene activation by RSK2, a growth factor-regulated CREB kinase. Science 273: 959 |
| 3. |
Xing, J., et al. Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation. Mol. Cell Biol. 18:1946-55 (1998). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
