Clone ID: Y106
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1495-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human
Mol. Weight: 58 kDa
UniProt ID: P14635
Alternative names:
Description: Cyclin B1 regulates mitosis in conjunction with Cdc2 kinase to form the Cdc2-cyclin B1 complex also known as maturation promoting factor (MPF) (1-2). The activation of the complex begins when the regulatory subunit, cyclin B1, associates with the catalytic subunit of Cdc2. Cyclin B1 levels rise during S phase and G2, and peak at mitosis. The timing of mitosis can also be influenced by changes in cyclin B1Cdc2 localization. Cyclin B1 is mostly located in the cytoplasm during S and G2 phases and then rapidly accumulates in the nucleus at the onset of prophase (3). Specificity: A synthetic peptide corresponding to residues in C-terminus of human cyclin B1 was used as immunogen. The antibody does not cross-react with other cyclin family members.
Recommended Dilutions:
WB: 1:5,000-20,000
IHC: 1:100
ICC: 1:50
Flow Cytometry: 1:100
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on (A) Hela cell lysate (B) Jurkat cell lysate using anti-Cyclin B1 RabMAb (catalog #1495-1), dilution 1:20,000. |
B. Immunohistochemical analysis of paraffin-embedded human skin carcinoma using anti-Cyclin B1 RabMAb (catalog #1495-1). |
Description References:
| 1. |
Morgan, D.O.. 1997. Cyclin-dependent kinases: engines, clocks, and microprocessors. Annu. Rev. Cell Dev. Biol. 13: 261-291 |
| 2. |
Dunphy, W.G.. 1994. The decision to enter mitosis. Trends Cell Biol. 4: 202-207 |
| 3. |
Gallant, P., and E.A. Nigg. 1992. Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells. J. Cell Biol. 117:213-224. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
