Clone ID: E296
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1492-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP, FC
Species Reactivity: Human, Mouse
Mol. Weight: 92 kDa
UniProt ID: P08238
Alternative names: HSP 84, HSP 90
Description: The 90-kDa heat shock protein (Hsp90) is a highly conserved, and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine and serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform , (Hsp90 α and Hsp90 β ) can be found in nearly equal amount in humans, and operates as part of a multichaperone machinery in the cytosol, which includes Hsp70, peptidyl-prolyl isomerases and other cochaperones (3). Specificity: A synthetic peptide corresponding to residues in N-terminus of human Hsp90 β was used as immunogen.
Recommended Dilutions:
WB: 1:500
IHC: 1:50
ICC: 1:50
Flow Cytometry: 1:10
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Hela cell lysate using anti-Hsp90 β (N-term) RabMAb (catalog #1492-1), dilution 1:500. |
B. Immunohistochemical analysis of paraffin-embedded urinary bladder carcinoma using anti-Hsp90 β (N-term) RabMAb (catalog #1492-1). |
Description References:
| 1. |
Lees-Miller S., Anderson C.W.; "Two human 90-kDa heat shock proteins are phosphorylated in vivo at conserved serines that are phosphorylated in vitro by casein kinase II.";J. Biol. Chem. 264:2431-2437(1989). |
| 2. |
Lees-Miller S., Anderson C.W.; "The human double-stranded DNA-activated protein kinase phosphorylates the 90-kDa heat-shock protein, hsp90 alpha at two NH2-terminal threonine residues."; J. Biol. Chem. 264:17275-17280(1989). |
| 3. |
Lotz G.P., Lin H., Harst A., Obermann W.M.J.; "Aha1 binds to the middle domain of Hsp90, contributes to client protein activation, and stimulates the ATPase activity of the molecular chaperone."; J. Biol. Chem. 278:17228-17235(2003). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
