Clone ID: E83-77
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1476-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse
Mol. Weight: 17 kDa
UniProt ID: P42574
Alternative names: CASP-3, Apopain, Cysteine protease CPP32, Yama protein, CPP-32, SREBP cleavage activity 1, SCA-1
Description: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3). Specificity: A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects the active form of caspase-3 (p17 subunit).
Recommended Dilutions:
WB: 1:500
IHC: 1:250
ICC: 1:100-250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis of Jurkat cell lysate using (A & B )anti-Pro-Caspase-3 RabMAb (catalog #1475-1), dilution 1:10,000 (C & D) anti-Caspase-3 RabMAb (catalog #1476-1), 1:500 dilution.
(A & C) Jurkat cell lysate (B & D) Jurkat cell lysate + Camptothecin |
B. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Caspase-3 RabMAb (catalog #1476-1). |
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| C. |
D. |
| Immunofluorescent staining of HeLa cells (C-control, D-staurosporine-treated) using anti-Caspase-3 RabMAb (catalog #1476-1). |
Product References:
| 1. | Haroon MF, Fatima A, Sch?ler S, et al, Minocycline, a possible neuroprotective agent in Leber?s hereditary optic neuropathy (LHON): Studies of cybrid cells bearing 11778 mutation ,Neurobiology of Disease 28 (2007) 237?250
[Application: WB] | | 2. | Ceder JA, Jansson L, Ehrnstrom RA, et al, The Characterization of Epithelial and Stromal Subsets of Candidate Stem/Progenitor Cells in the Human Adult Prostate ,European Urology 53 ( 2008) 524?532
[Application: ICC] | | 3. | Kiang JG et. al., Androstenediol inhibits the trauma-hemorrhage-induced increase in caspase-3 by downregulating the inducible nitric oxide synthase pathway ,J Appl Physiol, Nov 2006
[Application: WB] |
Description References:
| 1. |
Fernandes-Alnemri, T. et al. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J. Biol. Chem. 269:30761 |
| 2. |
Tewari, M. et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801 |
| 3. |
Nicholson, D.W. et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376: 37 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
