Clone ID: E83-103
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1475-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP, FC
Species Reactivity: Human, Mouse
Mol. Weight: 35 kDa
UniProt ID: P42574
Alternative names: CASP-3, Apopain, Cysteine protease CPP32, Yama protein, CPP-32, SREBP cleavage activity 1, SCA-1
Description: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3). Specificity: A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects pro-form (35kD) of caspase-3, and does not recognize any cleaved caspases.
Recommended Dilutions:
WB: 1:500
IHC: 1:250
ICC: 1:250
Flow Cytometry: 1:50
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis of (A) Jurkat cell lysate using anti-Pro-Caspase-3 RabMAb (B) Jurkat cell lysate + Camptothecin using anti-Pro-Caspase-3 RabMAb (catalog #1475-1), dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-3 RabMAb(catalog #1475-1). |
Product References:
| 1. | Kiang JG et. al., Androstenediol inhibits the trauma-hemorrhage-induced increase in caspase-3 by downregulating the inducible nitric oxide synthase pathway ,J Appl Physiol, Nov 2006
[Application: WB] |
Description References:
| 1. |
Fernandes-Alnemri, T. et al. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J. Biol. Chem. 269:30761 |
| 2. |
Tewari, M. et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801 |
| 3. |
Nicholson, D.W. et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376: 37 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
