Clone ID: Y69
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1472-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 57 kDa
UniProt ID: P01106
Alternative names: c-Myc, Transcription factor p64
Description: Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas (1), and the L-Myc gene in human small cell lung carcinomas (2). c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription (3). Specificity: A synthetic peptide corresponding to residues in N-terminus of human c-Myc was used as immunogen.
Recommended Dilutions:
WB: 1:10,000
IHC: 1:50
ICC: 1:250-500
IP: 1:150
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis of Jurkat cell lysate using anti-c-Myc (N-term) RabMAb (catalog #1472-1), dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded human skin carcinoma using anti-c-Myc (N-term) RabMAb (catalog #1472-1). |
|
|
| C. Immunofluorescent staining of HeLa cells using anti-c-Myc (N-term) RabMAb (catalog #1472-1). |
|
Description References:
| 1. |
Nisen, P.D. et al. Enhanced expression of the N-myc gene in Wilms |
| 2. |
Nau, M.N. et al. L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer. Nature 318: 69 |
| 3. |
Gupta, S. et al. Transactivation of Gene Expression by Myc is Inhibited by Mutation at the Phosphorylation Sites Thr-58 and Ser-62. Proc. Natl. Acad. Sci. USA 90: 3216 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
