Clone ID: E271
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1471-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC
Species Reactivity: Human
Mol. Weight: 50 kDa
UniProt ID: P32248
Alternative names: C-C CKR-7, CC-CKR-7, CCR-7, MIP-3 beta receptor, EBV-induced G-protein coupled receptor 1, EBI1, BLR2, CD197 antigen, CDw197
Description: CKR7, or C-C chemokine receptor type 7, is a G protein-coupled, seven-transmembrane domain receptor protein. Originally identified as the EBV-induced gene-1 (1), CKR7 was later found to be the receptor for the chemokines CCL21 (SLC, 6Ckine, Exodus-2) (3) and CCL19 (MIP-3?, Exodus-3, ELC) (4). CCR7 is expressed in lymphoid tissues and activated B and T lymphocytes (2). It is up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7 (4). Specificity: A synthetic peptide corresponding to residues near C-terminus of human CKR7 was used as immunogen. The antibody does not cross-react with other G-protein coupled receptor 1 family members. Predicted to cross-react with bovine, based on sequence homology.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:250
ICC: 1:250
Flow Cytometry: 1:20
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Daudi cell lysate anti-CCR7 (C-term) RabMAb (catalog #1471-1), dilution 1:1,000. |
B. Immunohistochemical analysis of paraffin-embedded normal liver tissue using anti-CCR7 (C-term) RabMAb (catalog #1471-1). |
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| C. Immunofluorescent staining of A431 cells using anti-CCR7 (C-term) RabMAb (catalog #1471-1). |
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Product References:
| 1. | Kodama J, Hasengaowa, Kusumoto T, et al, Association of CXCR4 and CCR7 chemokine receptor expression and lymph node metastasis in human cervical cancer ,Annals of Oncology 18 (2007) 70?76
[Application: IHC] | | 2. | Hasengaowa JK et. al., Association of CXCR4 and CCR7 chemokine receptor expression and lymph node metastasis in human cervical cancer ,Ann. Onc., Jan 2007; 18:70 - 76.
[Application: IHC] |
Description References:
| 1. |
Schweickart, V. L., et al. Cloning of human and mouse EBI1, a lymphoid-specific G-protein-coupled receptor encoded on human chromosome 17q12-q21.2. Genomics. 23: 643-50 (1994). |
| 2. |
Birkenbach, M., et al. Epstein-Barr virus-induced genes: first lymphocyte-specific G protein-coupled peptide receptors. J. Virol. 67: 2209-20 (1993). |
| 3. |
Yoshida, R., et al. Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7. J. Biol. Chem. 273: 7118-22 (1998). |
| 4. |
Yoshida, R., et al. Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7. J. Biol. Chem. 272: 13803-9 (1997). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
