Clone ID: Y92
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1469-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse
Mol. Weight: 190 kDa
UniProt ID: P09619
Alternative names: PDGF-R-beta, CD140b antigen
Description: Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-α and PDGF receptor-β. PDGF receptor-β can only bind two specific PDGF isoforms, PDGF-β and PDGF-Δ (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Specificity: A synthetic peptide corresponding to residues in C-terminus of human PDGF receptor- β was used as immunogen. The antibody does not cross-react with other CSF-1/PDGF receptor family members.
Recommended Dilutions:
WB: 1:10,000
IHC: 1:100
ICC: 1:100
IP: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on NIH 3T3 cell lysate using anti-PDGF receptor-ß (C-term) RabMAb (catalog #1469-1), dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded prostate carcinoma using anti-PDGF receptor-ß (C-term) RabMAb (catalog #1469-1). |
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| C. Immunofluorescent staining of NIH3T3 cells using anti-PDGF receptor-ß (C-term) RabMAb (catalog #1469-1). |
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Description References:
| 1. |
Deuel, T.F., et al. Platelet-derived growth factor: a multifunctional regulator of normal and abnormal cell growth. Biofactors 1: 213 |
| 2. |
Deuel, T.F. and J.S. Huang. Platelet-derived growth factor. Structure, function, and roles in normal and transformed cells. J Clin Invest. 74: 669 |
| 3. |
Kazlauskas, A. and J.A. Cooper. Autophosphorylation of the PDGF receptor in the kinase insert region regulates interactions with cell proteins. Cell 58: 1121 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
