Clone ID: E398P
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1399-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 500 kDa
UniProt ID: Q15149
Alternative names: PLTN, PCN, Hemidesmosomal protein 1, HD1, Plectin-11
Description: Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4). Specificity: A recombinant fragment comprising the C-terminal 220 amino acids of plectin was used as immunogen.
Recommended Dilutions:
WB: 1:5,000
IHC: 1:250
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A.
Western blot analysis on Jurkat cell lysate using anti-Plectin
RabMAb (catalog #1399-1), dilution 1:5,000. |
B. Immunohistochemical staining of paraffin-embedded human skeletal muscle using anti-Plectin RabMAb (catalog #1399-1). |
Description References:
| 1. |
Liu, C.G., et al. Human plectin: organization of the gene, sequence analysis, and chromosome localization (8q24). Proc Natl Acad Sci U S A. 93: 4278 |
| 2. |
Wiche, G. Role of plectin in cytoskeleton organization and dynamics. J. Cell. Sci. 111: 2477 |
| 3. |
Ruhrberg, C. and F.M. Watt. The plakin family: versatile organizers of cytoskeletal architecture. Curr. Opin. Genet. Dev. 7: 392 |
| 4. |
Foisner, R., et al. Protein kinase A and protein kinase C-regulated interaction of plectin with lamin B and vimentin. Proc. Natl. Acad. Sci. USA 88: 3812-3816 (1991). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
