Clone ID: E398L
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1398-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, ICC, IP
Species Reactivity: Human, Rat
Mol. Weight: 71 kDa
UniProt ID: Q14739
Alternative names: Integral nuclear envelope inner membrane protein, LMN2R
Description: Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization (1). Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures (2). The cleavage of lamins results in nuclear disregulation and cell death (3,4). Specificity: A synthetic peptide corresponding to residues of human Lamin B Receptor was used as immunogen.
Recommended Dilutions:
WB: 1:500
ICC: 1:500
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
 |
 |
| A. Western blot analysis on Jurkat cell lysate using anti-LBR RabMAb (cat. #1398-1), dilution 1:500. |
B. Immunofluorescent staining of HeLa cells using anti-LBR RabMAb (cat. #1398-1). |
Product References:
| 1. | Ulbert S, Antonin W, Platani M, et al, The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology ,FEBS Letters 580 (2006) 6435?6441
[Application: ICC] | | 2. | 119 J Cell Sci (21): 4442. (2006), Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins ,Maeshima et al.
[Application: IF] | | 3. | J. Cell Biol., Jan 2007; 176: 231 - 241., Novel cell death program leads to neutrophil extracellular traps ,Fuchs TA et. al.
[Application: EM, Gold] |
Description References:
| 1. |
Gruenbaum, Y., et al. Review: nuclear lamins |
| 2. |
Ye, Q. and H.J. Worman. Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane. J. Biol. Chem. 269: 11306 |
| 3. |
Oberhammer, F.A., et al. Chromatin condensation during apoptosis is accompanied by degradation of lamin A+B, without enhanced activation of cdc2 kinase. J. Cell Biol. 126: 827 |
| 4. |
Rao, L., et al. Lamin proteolysis facilitates nuclear events during apoptosis. J. Cell Biol. 135: 1441 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
