Clone ID: E173-58
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1326-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 17 kDa
UniProt ID: Q16695
Alternative names: H3t, H3/t, H3/g
Description: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5). Specificity: A synthetic peptide corresponding to residues surrounding Ser10 of Human Histone H3 was used as immunogen. Predicted to cross-react with most species, based on sequence homology.
Recommended Dilutions:
WB: 1:5,000-20,000
IHC: 1:100
ICC: 1:250
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis on Hela cell lysate using anti-Histone H3 RabMAb (cat. #1326-1), 1:20,000 dilution.
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B. Immunohistochemical staining of paraffin-embedded human cervical carcinoma using anti-Histone H3 RabMAb (cat. #1326-1). |
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C. Immunofluorescent staining of Hela cells using anti-Histone-3 RabMab(cat# 1326-1).
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Description References:
| 1. |
Braunstein, M., et al. Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern. Mol. Cell. Biol. 16: 4349 |
| 2. |
Workman, J.L. and R.E. Kingston. Alteration of nucleosome structure as a mechanism of transcriptional regulation. Annu. Rev. Biochem. 67: 545 |
| 3. |
Hansen, J.C. et al. Structure and function of the core histone N-termini: more than meets the eye. Biochemistry 37, 17637 |
| 4. |
Strahl, B.D. and C.D. Allis. The language of covalent histone modifications. Nature 403, 41 |
| 5. |
Hendzel, M.J., et al. Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 106: 348 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
