Clone ID: Y103
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1303-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse
Mol. Weight: 180 kDa
UniProt ID: P17948
Alternative names: VEGFR-1, Vascular permeability factor receptor, Tyrosine-protein kinase receptor FLT, Flt-1, Tyrosine-protein kinase FRT, Fms-like tyrosine kinase 1
Description: VEGFR-1 (vascular endothelial growth factor receptor 1) is a 180-kDa membrane receptor tyrosine kinase that is the receptor for vascular endothelial growth factor (VEGF), VEGFB and PGF. VEGF is a key regulator of physiological angiogenesis during embryogenesis, skeletal growth and reproductive functions. VEGF has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions (1). VEGFR-1 has seven extracellular Ig-like domains; the second domain from the N-terminus is necessary and sufficient for high affinity VEGF binding (2). The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2 (1), and two major tyrosine phosphorylation sites at Tyr 1213 and Tyr 1242 and two minor tyrosine phosphorylation sites at Tyr 1327 and Tyr 1333 exist in VEGFR-1 (3). Specificity: A synthetic peptide corresponding to residues in N-terminus of human VEGFR1 was used as immunogen. The antibody can detect both VEGFR1 and its splice isoform sFlt1.
Recommended Dilutions:
WB: 1:500 - 1000
IHC: 1:250
ICC: 1:250
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A.
Western blot analysis on mouse brain tissue using anti-VEGFR-1
(N-term) (cat. #1303-1); dilution 1:10,000. |
B.
Immunohistochemical analysis of paraffin-embedded skin cancer
using anti-VEGFR-1 (N-term) (cat. #1303-1). |
 |
|
| C.
Immunofluorescent staining of A431 cells using anti-VEGFR-1
(N-term) (cat. #1303-1). |
|
Product References:
| 1. | Cimpean AM, Raica M, Encicab S, et al, Immunohistochemical expression of vascular endothelial growth factor A (VEGF), and its receptors (VEGFR1, 2) in normal and pathologic conditions of the human thymus ,Ann Anat 190 (2008) 238-245
[Application: IHC] | | 2. | Am J Physiol Cell Physiol, May 2007, VEGF receptor inhibition blocks liver cyst growth in pkd2(WS25/-) mice ,Amura C et al
[Application: WB] |
Description References:
| 1. |
Ferrara, N. et al. The biology of VEGF and its receptors. Nat. Med. 9: 669 |
| 2. |
Starovasnik, M.A., et al. Solution structure of the VEGF-binding domain of Flt-1: comparison of its free and bound states. J Mol Biol. 293: 531 |
| 3. |
Ito, N., et al. Identification of vascular endothelial growth factor receptor-1 tyrosine phosphorylation sites and binding of SH2 domain-containing molecules. J Biol Chem. 273: 23410 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
