Clone ID: E302
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1302-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Rat
Mol. Weight: 60 kDa
UniProt ID: P30307
Alternative names: Dual specificity phosphatase Cdc25C
Description: Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C activates cell cycle-specific cyclin-dependent kinases (CDKs), including cdc2, via dephosphorylation events (1,2). Cdc25C is activated via phosphorylation at Ser216 (3). In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (4,5). Specificity: A synthetic peptide corresponding to residues near N-terminus of human Cdc25C was used as immunogen. The antibody can also detect splice isoform 2, 4 and 5 of human Cdc25C, based on sequence homology.
Recommended Dilutions:
WB: 1:5,000
IHC: 1:250-500
ICC: 1:250
IP: 1:80
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on HeLa cell lysate using anti-Cdc25C RabMAb (cat. #1302-1); dilution 1:5,000. |
B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti- Cdc25C RabMAb (cat. #1302-1). |
Description References:
| 1. |
Sadhu, K., et al. Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2. Proc Natl Acad Sci U S A. 87: 5139 |
| 2. |
Jessus, C. and R. Ozon. Function and regulation of cdc25 protein phosphate through mitosis and meiosis. Prog. Cell Cycle Res. 1: 215 |
| 3. |
Peng, C.Y., et al. Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216. Science 277: 1501 |
| 4. |
Blasina, A., et al. A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase. Curr Biol. 9: 1 |
| 5. |
Matsuoka, S., et al. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 282: 1893 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
