Clone ID: E277
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1277-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 62 kDa
UniProt ID: P19419
Alternative names:
Description: Elk-1 is a transcription factor that is part of the ets oncogene superfamily (1). Elk-1 forms a ternary complex with the serum response factors, SRE and SRF, mediating gene activity in response to serum and growth factors (2). Activation by several mitogen-activated protein kinases (Erk1 and Erk2) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli (3,4). Serines 383 and 389 in the C-terminal region are the preferred sites for phosphorylation-induced activation. Specificity: A synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen. The antibody does not cross-react with other ETS family members.
Recommended Dilutions:
WB: 1:500
IHC: 1:50
ICC: 1:100
IP: 1:80
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on A431 cell lysate using anti-Elk-1 (C-term) RabMAb (catalog #1277-1), dilution 1:500. |
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Product References:
| 1. | Mol. Cell. Biol., Feb 2007, Rev7/MAD2B links JNK pathway signalling to activation of the transcription factor Elk-1 ,Lin Zhang, Shen-Hsi Yang, and Andrew D. Sharrocks
[Application: WB] | | 2. | Mol. Cell. Biol., Apr 2007; 27: 2861 - 2869., Rev7/MAD2B Links c-Jun N-Terminal Protein Kinase Pathway Signaling to Activation of the Transcription Factor Elk-1 ,Lin Zhang, Shen-Hsi Yang, and Andrew D. Sharrocks
[Application: WB] |
Description References:
| 1. |
Rao, V.N., et al. elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints. Science 244: 66 |
| 2. |
Marais, R., et al. The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domain. Cell 73: 381 |
| 3. |
Harindranath, N., et al. The human elk-1 gene family: the functional gene and two processed pseudogenes embedded in the IgH locus. Gene 221: 215 |
| 4. |
Gille, H., et al. ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation. EMBO J. 14: 951 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
