Clone ID: E267
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1267-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 70 kDa
UniProt ID: P43403
Alternative names: 70 kDa zeta-associated protein, Syk-related tyrosine kinase
Description: Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3). Specificity: A synthetic peptide corresponding to residues in N-terminus of human ZAP-70 was used as immunogen.
Recommended Dilutions:
WB: 1:500
Flow Cytometry: 1:40
IP: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Jurkat cell lysate using anti-ZAP-70 RabMAb (cat. #1267-1); dilution 1:500. |
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Description References:
| 1. |
Chu, D.H., et al. The Syk family of protein tyrosine kinases in T-cell activation and development. Immunol. Rev. 165: 167 |
| 2. |
Isakov, N., et al. ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity. J Exp Med. 181: 375 |
| 3. |
Di Bartolo, V., et al. Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling. J Biol Chem. 274: 6285 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
