Clone ID: E249
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1249-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse
Mol. Weight: 22 kDa
UniProt ID: Q03135
Alternative names:
Description: Caveolins make up a family of proteins that are principal structural components of hairpin-like domains in the plasma membrane (1). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Three members of caveolins (caveolin-1, -2 and -3) have been identified, possessing different tissue distributions (2). Caveolins interact with multiple signaling molecules, such as the G-protein alpha subunit (1), tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (2,3). Caveolin-1 has been implicated in the pathogenesis of mammary epithelial cell hyperplasia (4). Specificity: A synthetic peptide corresponding to residues near C-terminus of human Caveolin-1 was used as immunogen. The antibody should recognize both alpha and beta form of Caveolin-1.
Recommended Dilutions:
WB: 1:20,000
IHC: 1:250
ICC: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
 |
 |
| A.
Immunohistochemical analysis of paraffin-embedded human urinary
bladder using anti-Caveolin-1 RabMAb (cat. #1249-1). |
B.
Immunohistochemical analysis of paraffin-embedded human lungs
using anti-Caveolin-1 RabMAb (cat. #1249-1). |
|
|
| C.
Western blot analysis on A431 cell lysate using anti-Caveolin-1
RabMAb (cat. #1249-1); dilution 1:20,000. |
|
Description References:
| 1. |
Glenney, Jr., J.R. The sequence of human caveolin reveals identity with VIP21, a component of transport vesicles. FEBS Lett. 314: 45 |
| 2. |
Okamoto, T., et al. Caveolins, a family of scaffolding proteins for organizing "preassembled signaling complexes" at the plasma membrane. J. Biol. Chem. 273: 5419 |
| 3. |
Smart, E.J., et al. Caveolins, liquid-ordered domains, and signal transduction. Mol. Cell. Biol. 19: 7289 |
| 4. |
Lee, H., et al. Caveolin-1 mutations (P132L and null) and the pathogenesis of breast cancer: caveolin-1 (P132L) behaves in a dominant-negative manner and caveolin-1 (-/-) null mice show mammary epithelial cell hyperplasia. Am J Pathol. 161: 1357 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
