Clone ID: E242
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1242-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 70 kDa
UniProt ID: P15336
Alternative names: Activating transcription factor 2, cAMP response element-binding protein CRE-BP1, HB16
Description: ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). Specificity: A synthetic peptide corresponding to residues N-terminus of human ATF-2 was used as immunogen. The antibody does not cross-react with other ATF family members.
Recommended Dilutions:
WB: 1:10,000
IHC: 1:100
ICC: 1:250-1:500
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on HeLa cell lysate using anti-ATF-2 RabMAb (cat. #1242-1); dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded human carcinoma using anti-ATF-2 RabMAb (cat. #1242-1).
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Description References:
| 1. |
Maekawa, T., et al. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO J. 8: 2023 |
| 2. |
Gupta, S., et al. Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science 267: 389 |
| 3. |
van Dam, H., et al. ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents. EMBO J. 14: 1798 |
| 4. |
Livingstone, C., et al. ATF-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J. 14: 1785 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
