Clone ID: Y77
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1235-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Rat
Mol. Weight: 45 kDa
UniProt ID: Q02750
Alternative names: MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1
Description: MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5). Specificity: A synthetic peptide corresponding to residues in C-terminus of human MEK1 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members.
Recommended Dilutions:
WB: 1:10,000
IHC: 1:250
ICC: 1:250-1:500
Flow Cytometry: 1:40
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A.
Western blot analysis on Jurkat cell lysate using anti-MEK1
(C-term) RabMAb (cat. #1235-1); dilution 1:10,000. |
B. Immunohistochemical analysis of urinary bladder carcinoma using anti-MEK1 (C-term) RabMAb (cat. #1235-1). |
Product References:
| 1. | Scholl FA, Dumesic PA, Barragan DI, et al, Mek1/2 MAPK Kinases Are Essential for Mammalian Development, Homeostasis, and Raf-Induced Hyperplasia ,Developmental Cell 12, 615?629, April 2007
[Application: IHC] |
Description References:
| 1. |
Crews, C. M., et al. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258: 478 |
| 2. |
Zheng, C.F., and K.L. Guan. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J Biol Chem. 268: 11435 |
| 3. |
Zheng, C.F., and K.L. Guan. Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. EMBO J. 13: 1123 |
| 4. |
Alessi, D.R., et al. Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1. EMBO J. 13: 1610 |
| 5. |
Rosen, L.B., et al. Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras. Neuron 12: 1207 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
