Clone ID: E208
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1208-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse
Mol. Weight: 90 kDa
UniProt ID: P42229
Alternative names:
Description: Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 is tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin (1,2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the a- and b-isoforms of Stat5 (3). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human Stat5 was used as immunogen. The antibody only detects Stat5 phosphorylated on Tyrosine 694.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:50
ICC: 1:50
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on A431 cell lysate using anti- Phospho-Stat5 (Y694) RabMAb (catalog #1208-1), dilution 1:1,000. Cell lysates were (1) untreated, (2) treated with EGF, or (3) treated with Phosphatase. |
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Phospho-Stat5 (Y694) RabMAb (catalog #1208-1). |
Product References:
| 1. | Blood, Mar 2007; 10.1182/blood-2007-01-069237., Different STAT-3 and STAT-5 phosphorylation discriminates among Ph-negative chronic myeloproliferative diseases and is independent of the V617F JAK-2 mutation ,Teofili L et. al.
[Application: IHC] |
Description References:
| 1. |
Hou, J., et al. Identification and purification of human Stat proteins activated in response to interleukin-2. Immunity 2: 321 |
| 2. |
Lin, J.X., et al. Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells. J. Biol Chem. 271: 10738 |
| 3. |
Litterst, C.M., et al. NCoA-1/SRC-1 is an essential coactivator of STAT5 that binds to the FDL motif in the alpha-helical region of the STAT5 transactivation domain. J Biol Chem. 278: 45340 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
