Clone ID: E184
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1184-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, IP, FC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 42 kDa
UniProt ID: P68032
Alternative names: α-cardiac actin
Description: Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4). Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Furthermore, expression of smooth muscle alpha actin is regulated by hormones and cell proliferation, and is altered by pathological conditions including oncogenic transformation and atherosclerosis (5). Specificity: A synthetic peptide corresponding to N-terminus of human Alpha-actin 3 was used as immunogen. This antibody only detects actin in smooth muscle in immunohistochemistry.
Recommended Dilutions:
WB: 1:50,000
IHC: 1:500
Flow Cytometry: 1:20
IP: 1:20
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
A.
Immunohistochemical analysis of paraffin-embedded human uterus
tissue using anti-a-Actin (Smooth
Muscle) RabMAb (cat. #1184-1); shows positive staining of smooth
muscle tissue.
|
B.
Immunohistochemical analysis of paraffin-embedded skeletal
muscle tissue using anti-a-Actin
(Smooth Muscle) RabMAb (cat. #1184-1); shows negative staining
of skeletal muscle tissue. |
|
|
| C.
Western blot analysis on A431 cell lysate using anti-a-Actin
(Smooth Muscle) RabMAb (catalog #1184-1), dilution 1:50,000. |
|
Product References:
| 1. | Hosack LW, Firpo MA, Scott JA, et al, Microvascular maturity elicited in tissue treated with cytokine-loaded hyaluronan-based hydrogels ,Biomaterials 29 (2008) 2336-2347
[Application: IHC] | | 2. | Zhan S et. al., Phagocytosis of apoptotic bodies by hepatic stellate cells induces NADPH oxidase and is associated with liver fibrosis in vivo ,Hepatology 43:435 - 443 (2006)
[Application: ICC] |
Description References:
| 1. |
Herman, I.M. Actin isoforms. Curr. Opin. Cell Biol. 5: 48 |
| 2. |
Bailly, M., et al. The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension. Curr Biol. 11: 620 |
| 3. |
Lim, Y.P., et al. Selective tyrosine hyperphosphorylation of cytoskeletal and stress proteins in primary human breast cancers: implications for adjuvant use of kinase-inhibitory drugs. Clin. Cancer Res. 10: 3980 |
| 4. |
Olson, T.M., et al. Actin mutations in dilated cardiomyopathy, a heritable form of heart failure. Science 280: 750 |
| 5. |
Miwa, T., et al. Structure, chromosome location, and expression of the human smooth muscle (enteric type) gamma-actin gene: evolution of six human actin genes. Mol Cell Biol. 11: 3296-306 (1991). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
