Clone ID: E180
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1180-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC
Species Reactivity: Human, Mouse
Mol. Weight: 34 kDa
UniProt ID: P55212
Alternative names: CASP-6, Apoptotic protease Mch-2
Description: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4). Specificity: A synthetic peptide corresponding to residues in C-terminus of human Caspase-6 was used as immunogen. The antibody should recognize both pro-form and p11 subunit.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:50
ICC: 1:100
Flow Cytometry: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A.
Western blot analysis on Jurkat cell lysate using anti- Caspase-6
RabMAb (cat. #1180-1); dilution 1:5,000. |
B.
Immunohistochemical analysis of paraffin-embedded human lung
adenocarcinoma using anti-Caspase-6 RabMAb (cat. #1180-1). |
|
|
| C.
Immunofluorescent staining of HeLa cells using anti-Caspase-6
RabMAb (cat. #1180-1). |
|
Description References:
| 1. |
Fernandes-Alnemri, T. et al. Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family. Cancer Res. 55: 2737 |
| 2. |
Srinivasula, S. M. et al. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J Biol. Chem. 271: 27099 |
| 3. |
Slee, E. A. et al. Ordering the Cytochrome c-initiated Caspase Cascade: Hierarchical Activation of Caspases-2, -3, -6, -7, -8, and -10 in a Caspase-9-dependent Manner. J. Cell Biol. 144: 281 |
| 4. |
Takahashi, A. et al. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis. Proc Natl Acad Sci U S A 93: 8395 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
