Clone ID: E175
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1175-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, IP
Species Reactivity: Human, Mouse
Mol. Weight: 70 kDa
UniProt ID: P23443
Alternative names: Ribosomal protein S6 kinase I, S6K, S6K1, 70 kDa ribosomal protein S6 kinase 1, p70 S6 kinase alpha, p70(S6K)-alpha, p70-S6K, P70S6K, p70-alpha
Description: p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (2). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Thr389 of human p70 S6 kinase was used as immunogen. The antibody only detects p70 S6 kinase phosphorylated on Threonine 389. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.
Recommended Dilutions:
WB: 1:1,000-1:5,000
IHC: 1:50
IP: 1:60
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A.
Western blot analysis on Jurkat cell lysate using anti-Phospho-p70
S6 Kinase (pT389) RabMAb (cat. #1175-1); dilution 1:1,000. Cells
were either (1) serum-starved or (2) treated with FBS. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-p70 S6 Kinase (pT389) RabMAb (cat. #1175-1). |
Product References:
| 1. | Mol. Cancer Ther., Oct 2006; 5: 2494 - 2502., Signaling interactions of rapamycin combined with erlotinib in cervical carcinoma xenografts ,Diana C. Birle and David W. Hedley |
Description References:
| 1. |
Pearson, R.B. and G. Thomas. Regulation of p70s6k/p85s6k and its role in the cell cycle. Prog Cell Cycle Res. 1: 21 |
| 2. |
Pullen, N. and G. Thomas. The modular phosphorylation and activation of p70s6k. FEBS Lett. 410: 78 |
| 3. |
Dufner, A. and G. Thomas. Ribosomal S6 kinase signaling and the control of translation. Exp Cell Res. 253: 100 |
| 4. |
Le, X.F., et al. Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis. Oncogene 22: 484 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
