Clone ID: E161
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1161-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 34 kDa
UniProt ID: P06493
Alternative names: EC 2.7.11.23, p34 protein kinase, Cyclin-dependent kinase 1, CDK1
Description: During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4). Specificity: A synthetic peptide corresponding to residues surrounding Tyr15 of human Cdc2 was used as immunogen. The antibody detects Cdc2 without phosphorylation on Tyrosine 15. It does not detect Y15-phosphorylated Cdc2. Predicted to cross-react with bovine, based on sequence homology.
Recommended Dilutions:
WB: 1:2000 - 5000
IHC: 1:50
ICC: 1:500
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on Hela cell lysate using anti-Cdc2 (Y15) RabMAb (catalog #1161-1), 1:5,000 dilution. Cell lysates were (1) untreated, (2) treated with Hydroxyurea, and (3) treated with Phosphatase. |
B. Immunohistochemical staining of paraffin-embedded human lymphoma using anti-cdc2 RabMab (catalog #1161-1). |
Description References:
| 1. |
Norbury, C. and P. Nurse. Animal cell cycles and their control. Annu. Rev. Biochem. 61: 441 |
| 2. |
Atherton-Fessler, S. et al. Mechanisms of p34cdc2 regulation. Mol. Cell. Biol. 13: 1675 |
| 3. |
Watanabe, N. et al. Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle. EMBO J. 14: 1878 |
| 4. |
Galaktionov, K. et al. Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation. Genes Dev. 9: 1046 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
