Clone ID: E155
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1155-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Rat
Mol. Weight: 36 kDa
UniProt ID: P67775
Alternative names: PP2A-alpha, Replication protein C, RP-C
Description: Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways (1-3). PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A (4). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Tyr307 of human PP2A was used as immunogen. The antibody only detects PP2A phosphorylated on Tyrosine 307. Predicted to cross-react with mouse, rat, bovine and pig, based on sequence homology.
Recommended Dilutions:
WB: 1:5,000
IHC: 1:50
ICC: 1:50
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A.
Western blot analysis on A431 cell lysate using anti-Phospho-PP2A
(Y307) RabMAb (catalog #1155-1), dilution 1:5,000. Cell lysates
were either (1) untreated or (2) treated with EGF. |
B. Immunohistochemical analysis of paraffin-embedded human brain using anti-Phospho-PP2A (Y307) RabMAb (catalog #-1155-1). |
Product References:
| 1. | Mart?n-Encabo S, Santos E, Guerrero C, C3G mediated suppression of malignant transformation involves activation of PP2A phosphatases at the subcortical actin cytoskeleton ,Experimental cell reserarch 313 (2007) 3881-3891
[Application: WB, IP] | | 2. | Chen L, Liu L, Huang SL, et al, Cadmium activates MAPK pathway via induction of reactive oxygen species and inhibition of protein phosphatase 2A and 5 ,Free Radical Biology and Medicine (2008), Accepted Manuscript
[Application: WB] | | 3. | Nevlani P, Santhanam S, Trotta R, et al, The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABLregulated SET protein ,Cancer Cell 8 (2005) 355-368
[Application: WB, IP] | | 4. | Mol. Pharmacol., Feb 2007; 10.1124/mol.106.033555., Domains Necessary for G{alpha}12 Binding and Stimulation of PP2A: is G{alpha} 12 a Novel Regulatory Subunit of PP2A? ,Zhu D et. al.
[Application: WB] |
Description References:
| 1. |
Janssens, V. and J. Goris. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J. 353: 417 |
| 2. |
Zabrocki, P., et al. Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol. Microbiol. 43: 835 |
| 3. |
Milward, T.A., et al. Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem. Sci. 24: 186 |
| 4. |
Chen, J., et al. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science 257: 1261-1264 (1992). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
