Clone ID: V2
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1149-1
Size: 100ul
Price: $ 285
Recommended Applications: IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 180 kDa
UniProt ID: P04626
Alternative names: p185erbB2, C-erbB-2, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, MLN 19, CD340 antigen
Description: HER2 / ErbB2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. Specificity: A synthetic peptide corresponding to residues of human ErbB2 was used as immunogen.
Recommended Dilutions:
IHC: 1:500
ICC: 1:500
IP: 1:80
Protocols for using Epitomics Rabbit Monoclonal Antibodies
 |
 |
| A.
Immunohistochemical staining of paraffin-embedded human breast
ductal carcinoma in situ using anti-ErbB2/HER2 RabMAb (cat.
#1149-1). |
B.
Immunohistochemical staining of paraffin-embedded human breast ductal carcinoma in situ using anti-ErbB2/HER2 RabMAb (cat. #1149-1). |
Description References:
| 1. |
Muthuswamy, S. K., et al. Controlled dimerization of ErbB receptors provides evidence for differential signaling by homo- and heterodimers. Mol. Cell. Biol. 19: 6845-6857 (1999). |
| 2. |
Qian, X., et al. Heterodimerization of epidermal growth factor receptor and wild-type or kinase-deficient Neu: a mechanism of interreceptor kinase activation and transphosphorylation. Proc. Natl. Acad. Sci. USA 91: 1500 |
| 3. |
Dittadi, R. and M. Gion. More about: prognostic importance of low c-erbB2 expression in breast tumors. J. Natl. Cancer Inst. 92: 1443-1444 (2000). |
| 4. |
Klapper, L. N., et al. Tumor-inhibitory antibodies to HER-2/ErbB-2 may act by recruiting c-Cbl and enhancing ubiquitination of HER-2. Cancer Res. 60: 3384-3388 (2000). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
