Clone ID: E144
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1144-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 39 kDa
UniProt ID: P16422
Alternative names: Major gastrointestinal tumor-associated protein GA733-2, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Adenocarcinoma-associated antigen, KSA, KS 1/4 antigen, Cell surface glycoprotein Trop-1, CD326 antigen
Description: Epithelial cell adhesion molecule (Ep-CAM) is a monomeric membrane glycoprotein that is expressed on virtually all epithelial cell membranes and on a variety of epithelial carcinomas (1-3). Ep-CAM is overexpressed in a variety of human carcinomas and is, therefore, a potential therapeutic target for human solid tumors (4). Ep-CAM contains an extracellular domain with two epidermal growth factor-like repeats, followed by a cysteine-poor region, which are necessary for the adhesion properties of the molecule (5). Specificity: A synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human Ep-CAM was used as immunogen.
Recommended Dilutions:
WB: 1:500 - 1000
IHC: 1:100-1:250
ICC: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A.
Western blot analysis on A431 cell lysate using anti-Epithelial
Antigen (Ep-CAM) (C-term) RabMAb (cat. #1144-1); dilution 1:2,500. |
B.
Immunohistochemical analysis of paraffin-embedded stomach carcinoma
using anti-Epithelial Antigen (Ep-CAM) (C-term) RabMAb (cat.
#1144-1). |
Description References:
| 1. |
Tomita, Y., et al. Molecular identification of a human carcinoma-associated glycoprotein antigen recognized by mouse monoclonal antibody FU-MK-1. J. Cancer Res. 91: 231 |
| 2. |
Trebak, M., et al. Oligomeric state of the colon carcinoma-associated glycoprotein GA733-2 (Ep-CAM/EGP40) and its role in GA733-mediated homotypic cell-cell adhesion. J. Biol. Chem. 276: 2299 |
| 3. |
Simon, B., et al. Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Proc Natl Acad Sci U S A. 87: 2755 |
| 4. |
Szala, S., et al. Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2. Proc Natl Acad Sci U S A. 87: 3542 |
| 5. |
Balzar, M., et al. Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions. Mol. Cell. Biol. 21: 2570 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
