Clone ID: E142
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1142-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC
Species Reactivity: Human, Rat
Mol. Weight: 50 kDa
UniProt ID: O96020
Alternative names:
Description: Cyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathway(s) during this phase (1). Cyclin E2 specifically interacts with Cdk inhibitors of the CIP/KIP family and activates both Cdk2 and Cdk3. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both (2). Abnormally high levels of cyclin E expression have frequently been observed in human cancers (3). Specificity: A synthetic peptide corresponding to residues in C-terminus of human Cyclin E2 was used as immunogen. Predicted to cross-react with mouse and bovine, based on sequence homology.
Recommended Dilutions:
WB: 1:500
IHC: 1:50
ICC: 1:100-1:250
Flow Cytometry: 1:20
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A.
Western blot analysis on HeLa cell lysate using anti-Cyclin
E2 (C-term) RabMAb (cat. #1142-1); dilution 1:500. |
B. Immunohistochemical
analysis of paraffin-embedded human breast carcinoma using anti-Cyclin
E2 (C-term) RabMAb (cat. #1142-1). |
|
|
| C.
Immunofluorescent staining of HeLa cells using anti-Cyclin E2
(C-term) RabMAb (cat. #1142-1). |
|
Product References:
| 1. | Laurent Sansregret et.al, The p110 Isoform of the CDP/Cux Transcription Factor Accelerates Entry into S Phase ,Mol. Cell. Biol., Mar 2006; 26: 2441 - 2455
[Application: WB] |
Description References:
| 1. |
Lauper, N., et al. Cyclin E2: a novel CDK2 partner in the late G1 and S phases of the mammalian cell cycle. Oncogene 17: 2637 |
| 2. |
Zariwala, M., et al. Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Oncogene 17: 2787 |
| 3. |
Gudas, J.M., et al. Cyclin E2, a novel G1 cyclin that binds Cdk2 and is aberrantly expressed in human cancers. Mol Cell Biol. 19: 612 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
