Clone ID: E130
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1130-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 35 kDa
UniProt ID: P25963
Alternative names: Major histocompatibility complex enhancer-binding protein MAD3, I-kappa-B-alpha, IkappaBalpha, IKB-alpha
Description: The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein (1-4). In response to many different NF-kappaB-inducing agentsincluding T-cell mitogens, proinflammatory cytokines, and viral transactivatorsthe inhibitory IkappaB-alpha is rapidly phosphorylated and degraded (2). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha (3). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism (1-4). Specificity: A synthetic peptide corresponding to human IkappaB-alpha was used as immunogen. Predicted to cross-react with pig, bovine, mouse and rat, based on sequence homology.
Recommended Dilutions:
WB: 1:10,000
IHC: 1:50 - 1:100
ICC: 1:100
IP: 1:25
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A.
Western blot analysis of Hela cell lysate using anti-IkappaB-alpha RabMAb (catalog #1130-1), 1:10,000 dilution. |
B.
Immunohistochemical analysis of paraffin-embedded human
prostate carcinoma using anti-IkappaB-alpha RabMAb
(catalog #1130-1). |
 |
|
| C.
Flow cytometric analysis of permeabilized HeLa cells, untreated
(green), TNF-a-treated (red), using anti-IkappaB-alpha
RabMAb (catalog #1130-1). |
|
Product References:
| 1. | MartinezO, Valmas C, Basler CF, Ebola virus-like particle-induced activation of NF-?B and Erk signaling in human dendritic cells requires the glycoprotein mucin domain ,Virology 364 (2007) 342?354
[Application: WB] |
Description References:
| 1. |
Traenckner, E.B., et al. Phosphorylation of human I kappa B-alpha on serines 32 and 36 controls I kappa B-alpha proteolysis and NF-kappa B activation in response to diverse stimuli. EMBO J. 14: 2876 |
| 2. |
Brockman, J.A., et al. Coupling of a signal response domain in I kappa B alpha to multiple pathways for NF-kappa B activation. Mol. Cell. Biol. 15: 2809 |
| 3. |
Chen, Z.J., et al. Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity. Cell 84: 853 |
| 4. |
Brown, K., et al. Control of I kappa B-alpha proteolysis by site-specific, signal-induced phosphorylation. Science 267: 1485 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
