Clone ID: E121-21
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1122-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 92 kDa
UniProt ID: P40763
Alternative names: Acute-phase response factor
Description: Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727 (4). Specificity: A synthetic peptide corresponding to residues surrounding Ser727 of human Stat3 was used as immunogen. The antibody only detects Stat3 without phosphorylation on Serine 727. It does not detect S727-phosphorylated Stat3. Predicted to cross-react with bovine, and horse, based on sequence homology.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:50
ICC: 1:100
IP: 1:20
Protocols for using Epitomics Rabbit Monoclonal Antibodies
 |
 |
| A. Western
blot analysis on A431 cell lysate using anti-Stat3 RabMAb (catalog #1122-1), 1:1,000 dilution. |
B.
Immunohistochemical analysis of paraffin-embedded lung squamous
carcinoma using anti-Stat3 RabMAb (catalog #1122-1). |
Product References:
| 1. | Hum. Mol. Genet., Jan 2006, Stem-cell protein Piwil2 is widely expressed in tumors and inhibits apoptosis through activation of Stat3/Bcl-XL pathway ,Lee JH et al
[Application: WB] |
Description References:
| 1. |
Heim, M.H. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19: 75 |
| 2. |
Kisseleva, T. et al. Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene 285: 1 |
| 3. |
Bromberg, J.F. et al. Stat3 as an Oncogene. Cell 98: 295 |
| 4. |
Wen, Z. et al. Maximal activation of transcription by statl and stat3 requires both tyrosine and serine phosphorylation. Cell 82: 241 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
