Clone ID: E90
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1090-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 36 kDa
UniProt ID: P05198
Alternative names: Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, EIF-2alpha, EIF-2A
Description: Eukaryotic initiation factor 2 (eIF2) plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. Phosphorylation of a-subunit of eIF2 effectively prevents formation of the eIF2/GTP/Met-tRNAi complex and inhibits global protein synthesis (1-3). Three distinct protein kinases regulate protein synthesis in eukaryotic cells by phosphorylating the a-subunit of eIF2 at Serine51 (3). Phosphorylation occurs under a wide variety of different stimuli, including heat shock, serum deprivation, glucose starvation, amino acid starvation, exposure to heavy metal ions, and viral infection (3). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a was used as immunogen. The antibody only detects eIF-2a phosphorylated on Serine 51. Predicted to cross-react with mouse, rat, bovine and pig, based on sequence homology.
Recommended Dilutions:
WB: 1:500-1,000
IHC: 1:50-1:100
ICC: 1:100-1:250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A.
Western blot analysis on PC12 cell lysate using anti- Phospho-eIF-2alpha (pS51) RabMAb (cat. #1090-1); dilution 1:1,000. A: non treated cells B: treated with NGF C: treated with Pervanadate D: treated with FBS |
B.
Immunohistochemical analysis of paraffin-embedded human liver
carcinoma using anti-Phospho-eIF-2alpha (pS51) RabMAb (cat.
#1090-1). |
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| C. Dot Blot analysis using anti-Phospho eIF2alpha (pS51) (cat#1090-1) against eIF2alpha peptides (A) phosphorylated at Serine 51 and (B) non-phosphorylated peptide. |
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Product References:
| 1. | C. Deval, J. Talvas, C. Chaveroux, et al, Amino acid limitation induces the GCN2 signaling pathway in myoblasts but not in myotubes ,Biochimie (2008) in press
[Application: WB] | | 2. | Lee ES, Yoon CH, Kim YS, et al, The double-strand RNA-dependent protein kinase PKR plays a significant role in a sustained ER stress-induced apoptosis ,FEBS Letters 581 (2007) 4325?4334
[Application: ICC] | | 3. | J. Biol. Chem., Jan 2007; 282:240 - 248., Hypoxic Regulation of Id-1 and Activation of the Unfolded Protein Response Are Aberrant in Neuroblastoma ,S. Maureen Nemetski and Lawrence B. Gardner
[Application: WB] | | 4. | Am J Physiol Cell Physiol, May 2007; 292: C1971 - C1981., Domains Necessary for G Alpha?12Binding and Stimulation of Protein Phosphatase-2A (PP2A): Is G Alpha?12a Novel Regulatory Subunit of PP2A? ,Cordas E et al
[Application: IHC] |
Description References:
| 1. |
Ernst, H., et al. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA. J Biol Chem. |
| 2. |
Kimball, S.R. Eukaryotic initiation factor eIF2. Int. J. Biochem. Cell Biol. 31: 25 |
| 3. |
De Haro, C., et al. The eIF-2alpha kinases and the control of protein synthesis. FASEB J. 10: 1378 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
