Clone ID: E84
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1084-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, FC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 46 kDa
UniProt ID: P55211
Alternative names: CASP-9, ICE-like apoptotic protease 6, ICE-LAP6, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3
Description: Caspase-9 (ICE-LAP6, Mch-6, Apaf-3) is a member of the cysteine aspartic acid protease, or caspase, family. Pro-Caspase-9 exists as a 46 kDa isomer that is cleaved by apoptotic stimulation, and functions as the major initiator caspase in programmed cell death, or apoptosis (1-4). Upon stimulation, cytochrome c is released from mitochondria, and associates with the Pro-Caspase-9/Apaf 1 complex. This complex in turn activates Pro-Caspase-9 by cleaving it into an active 35 kDa fragment and a small 10 kDa fragment (1,2). Activated Caspase-9 cleaves Caspase-3 as well as poly (ADP-ribose) polymerase (PARP) to initiate a caspase cascade and apoptosis (3,4). Specificity: A synthetic peptide corresponding to residues near N-terminus of human Caspase-9 was used as immunogen. This antibody only recognises the Pro form of Caspase-9 (46kDa).
Recommended Dilutions:
WB: 1:500-2000
IHC: 1:25
Flow Cytometry: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western
blot analysis on Jurkat cell lysate using anti-Pro-Caspase-9 RabMAb (catalog #1084-1), 1:1,000 dilution. |
B. Immunohistochemical
analysis of paraffin-embedded human colon adenocarcinoma using
anti-Pro-Caspase-9 RabMAb (catalog #1084-1). |
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| C. Flow cytometric analysis of permeabilized HeLa cells using anti-Pro-Caspase-9 RabMAb (red) (catalog # 1084-1) or a rabbit IgG (negative) (green).
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Product References:
| 1. | Kiang JG et. al., Androstenediol inhibits the trauma-hemorrhage-induced increase in caspase-3 by downregulating the inducible nitric oxide synthase pathway ,J Appl Physiol, Nov 2006
[Application: WB] | | 2. | J Appl Physiol, May 2007; 10.1152, Geldanamycin inhibits hemorrhage-induced increases in caspase-3 activity: Role of inducible nitric oxide synthase ,Kiang JG et al.
[Application: WB] |
Description References:
| 1. |
Kuida, K. et al. Reduced Apoptosis and Cytochrome c |
| 2. |
Li, P. et al. Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade. Cell 91: 479 |
| 3. |
Duan, H. et al. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 271: 16720 |
| 4. |
Srinivasula, S.M. et al. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J. Biol. Chem. 271: 27099 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
