Clone ID: Y89
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1081-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, ICC, IP, FC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 59 kDa
UniProt ID: P31749
Alternative names: RAC-PK-alpha, Protein kinase B, PKB, C-AKT
Description: Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified. Specificity: A synthetic peptide corresponding to residues in C-terminus of human Akt1 was used as immunogen. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.
Recommended Dilutions:
WB: 1:5,000-10,000
IHC: 1:100
ICC: 1:100-1:250
Flow Cytometry: 1:20
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on MCF-7 cell lysate using anti- Akt1/PKBa (C-term) RabMAb (cat. #1081-1); dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded prostate carcinoma using anti-Akt1/PKBa (C-term) RabMAb (cat. #1081-1). |
Product References:
| 1. | Goren I, M?ller E, Pfeilschifter J, et al, Thr308 determines Akt1 nuclear localization in insulin-stimulated keratinocytes ,Biochemical and Biophysical Research Communications 372 (2008) 103?107
[Application: WB, IP] |
Description References:
| 1. |
Burgering, B.M. and P.J. Coffer. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376: 599 |
| 2. |
Cardone, M.H., et al. Regulation of cell death protease caspase-9 by phosphorylation. Science 282: 1318 |
| 3. |
Hajduch, E., et al. Protein kinase B (PKB/Akt)--a key regulator of glucose transport? FEBS Lett. 492: 199 |
| 4. |
Cross, D.A., et al. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 378: 785 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
