Clone ID: E102
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1072-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC
Species Reactivity: Human, Rat
Mol. Weight: 116/25 kDa
UniProt ID: P09874
Alternative names: PARP-1, ADPRT, NAD(+) ADP-ribosyltransferase 1, Poly[ADP-ribose] synthetase 1
Description: Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA
binding protein that recognizes DNA strand breaks and is presumed to
play a role in DNA repair (1). As a marker for apoptosis, PARP is
cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing
as a 116 kDa nuclear protein, PARP is cleaved between amino acids
Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic
domain) and 85 kDa (N-terminal DNA-binding domain) (2,4). Specificity: A synthetic peptide corresponding to N-terminal residues
of human PARP-1 was used as immunogen. The antibody should recognize
both pro-form and p25 cleaved-form of PARP-1.
Recommended Dilutions:
WB: 1:500 - 1,000
IHC: 1:25
ICC: 1:100
Flow Cytometry: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Ikejima, M, et al. The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA. J.Biol.Chem. 265: 21907 |
| 2. |
Lazebnik, Y.A., et al. Cleavage of poly(ADP-ribose) polymerase by a
proteinase with properties like ICE. Nature 371: 346 |
| 3. |
Tewari, M, et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is
a CrmA-inhibitable protease that cleaves the death substrate
poly(ADP-ribose) polymerase. Cell 81: 801 |
| 4. |
Kaufmann, S.H., et al. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: An early marker of chemotherapy-induced apoptosis. Cancer Res. 53: 2976 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
