Clone ID: E63
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1063-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 21 kDa
UniProt ID: Q07814
Alternative names:
Description: Bax is a pro-apoptotic member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bax promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bax translocates from the cytosol to the mitochondrial membrane (2). Through interactions with mitochondrial membrane proteins, Bax increases the membrane's permeability, leading to the release of cytochrome c from mitochondria and initiation of the apoptotic caspase cascade (2,3). Specificity: A synthetic peptide corresponding to residues in the vicinity of the dimerization domain of human Bax was used as immunogen. The antibody should recognize cytoplasmic isoform beta, delta, and membrane isoform alpha of Bax. The antibody does not cross-react with other Bcl-2 family members. Predicted to cross-react with bovine based on sequence homology.
Recommended Dilutions:
WB: 1:1,000 - 2,000
IHC: 1:250
ICC: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on HeLa cell lysate using anti-Bax RabMAb (cat. #1063-1), 1:2,000 dilution. |
B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti-Bax RabMAb (cat. #1063-1). |
Product References:
| 1. | Swayze EE, Siwkowski AM, Wancewicz EV, et al, Antisense oligonucleotides containing locked nucleic acid improve potency but cause significant hepatotoxicity in animals ,Nucleic Acids Research 35 (2007) 687?700
[Application: IHC] | | 2. | Swayze EE et. al., Antisense oligonucleotides containing locked nucleic acid improve potency but cause significant hepatotoxicity in animals ,Nucleic Acids Research, 2007, 35(2):687-700
[Application: IHC] | | 3. | Eliseev RA et. al., Increased radiation-induced apoptosis of Saos2 cells via inhibition of NFB: A role for c-Jun N-terminal kinase ,Journal of Cellular Biochemistry 96:1262-1273 (2005).
[Application: WB] |
Description References:
| 1. |
Oltvai, Z.N., et al. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74: 609 |
| 2. |
Jurgensmeier, J.M., et al. Bax directly induces release of cytochrome c from isolated mitochondria. Proc. Natl. Acad. Sci. USA 95: 4997 |
| 3. |
Narita, M., et al. Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria. Proc. Natl. Acad. Sci. USA 95: 14681 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
