Clone ID: Y62
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1062-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 30 kDa
UniProt ID: P31946
Alternative names: Protein kinase C inhibitor protein 1, KCIP-1, Protein 1054
Description: The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3). Specificity: A synthetic peptide corresponding to C-terminal residues of human 14-3-3 /a was used as immunogen.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:500
ICC: 1:500
Flow Cytometry: 1:40
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis on Hela cell lysate using anti-14-3-3 ß/a (C-term) RabMAb (cat. #1062-1), dilution 1:1,000.
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B. Immunohistochemical analysis of paraffin-embedded breast tissue anti-14-3-3 ß/a (C-term) RabMAb (cat. #1062-1). |
Description References:
| 1. |
Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617 |
| 2. |
Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889 |
| 3. |
Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
