Clone ID: E58
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1058-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, FC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 30 kDa
UniProt ID: P31946
Alternative names: Protein kinase C inhibitor protein 1, KCIP-1, Protein 1054
Description: The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3) Specificity: A synthetic peptide corresponding to residues near N-terminus of human 14-3-3 beta/alpha was used as immunogen. Predicted to cross-react with sheep and bovine, based on sequence homology.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:250
Flow Cytometry: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
A. Western
blot analysis of HeLa cell lysate probed with anti-14-3-3
RabMab (Cat#1058-1).
|
B. Immunohistochemical
analysis of human skin using anti-14-3-3 RabMab (cat#1058-1). |
 |
|
| C. Flow
cytometric analysis of permeabilized HeLa cells using anti-14-3-3_/_
RabMAb (red) (catalog #1058-1) or a rabbit IgG (negative) (green). |
|
Description References:
| 1. |
Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617 |
| 2. |
Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889 |
| 3. |
Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
