Clone ID: E54
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1054-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP, FC
Species Reactivity: Human
Mol. Weight: 110 kDa
UniProt ID: Q96EB6
Alternative names: hSIRT1, hSIR2, SIR2-like protein 1
Description: The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4). Specificity: A synthetic peptide corresponding to residues near N-terminus and before Ala-rich domain of human Sir2 was used as immunogen. The antibody does not cross-react with other sirtuin family members.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:50
ICC: 1:100
Flow Cytometry: 1:30
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis on Hela cell lysate using anti-Sir2 RabMAb (catalog #1054-1), dilution 1:1,000.
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B. Immunohistochemical staining of paraffin-embedded human lymphoma using anti-Sir2 RabMAb (catalog #1054-1). |
Description References:
| 1. |
David A. Sinclair et al; Inhibition of Silencing and Accelerated Aging by Nicotinamide, a Putative Negative Regulator of Yeast Sir2 and Human SIRT1*; J. Biol. Chem., Vol. 277, Issue 47, 45099-45107, November 22, 2002 |
| 2. |
Smith, J. S., Brachmann, C. B., Celic, I., Kenna, M. A., Muhammad, S., Starai, V. J., Avalos, J. L., Escalante-Semerena, J. C., Grubmeyer, C., Wolberger, C., and Boeke, J. D. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 6658-6663 |
| 3. |
Kouzarides et al, Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence; The EMBO Journal, Vol. 21, No. 10 pp. 2383-2396, 2002 |
| 4. |
Tanner, K. G., Landry, J., Sternglanz, R., and Denu, J. M. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 14178-14182 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
