Clone ID: E51
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1051-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse
Mol. Weight: 25 kDa
UniProt ID: P09874
Alternative names: PARP-1, ADPRT, NAD(+) ADP-ribosyltransferase 1, Poly[ADP-ribose] synthetase 1
Description: Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4). Specificity: A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognize p25 cleaved-form of PARP-1.
Recommended Dilutions:
WB: 1:50,000 - 100,000
IHC: 1:100
ICC: 1:250
Flow Cytometry: 1:50
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Jurkat cell lysate using anti-PARP-1 Cleaved (p25) RabMAb (cat. #1051-1), 1:50,000 dilution. Cells were either (1) untreated or (2) treated with Camptothecin. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PARP-1 (Cleaved p25) RabMAb (cat. #1051-1). |
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| C. Immunofluorescent staining of Hela cell using anit-PARP-1 RabMab (cat# 1051-1) |
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Product References:
| 1. | El-Domyati MM, Al-Din ABM, Barakat MT, et al, Deoxyribonucleic acid repair and apoptosis in testicular germ cells of aging fertile men: the role of the poly(adenosine diphosphate-ribosyl)ation pathway ,Fertility and Sterility (2008) in press
[Application: IHC] | | 2. | Simonssona M, Qvarnstro?ma F, Nymanb J,et al, Low-dose hypersensitive cH2AX response and infrequent apoptosis in epidermis from radiotherapy patients ,Radiother Oncol (2008), Original article
[Application: IHC] |
Description References:
| 1. |
Ikejima, M, et al. The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA. J.Biol.Chem. 265: 21907 |
| 2. |
Lazebnik, Y.A., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371: 346 |
| 3. |
Tewari, M, et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801 |
| 4. |
Kaufmann, S.H., et al. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: An early marker of chemotherapy-induced apoptosis. Cancer Res. 53: 2976 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
