Clone ID: E40
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1040-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Rat
Mol. Weight: 72 kDa
UniProt ID: Q13489
Alternative names: Inhibitor of apoptosis protein 1, HIAP1, HIAP-1, C-IAP2, TNFR2-TRAF-signaling complex protein 1, IAP homolog C, Apoptosis inhibitor 2, API2, RING finger protein 49
Description: cIAP-2, also known as HIAP-1, is a member of the inhibitor of apoptosis (IAP) family of proteins. These proteins contain a BIR (baculovirus IAP repeat) domain near the amino-terminus (1). cIAP-2 is localized to the cytoplasm. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form a heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). This complex is thought to play a role in inhibition of apoptosis (2,3). cIAP-2 inhibits cytochrome c-induced activation of caspase-9, thereby preventing initiation of the caspase cascade and apoptosis (4). Specificity: A synthetic peptide corresponding to residues, before the breakpoint for translocation to form BIRC3-MALT1, of Human BIP3 was used as immunogen. The antibody does not cross-react with other IAP family members.
Recommended Dilutions:
WB: 1:500
IHC: 1:50
ICC: 1:100
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis on Daudi cell lysate using anti-HIAP-1/BIR3 RabMAb (catalog #1040-1), dilution 1:1,000.
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B. Immunohistochemical analysis of human normal spleen using anti-HIAP-1/BIR3 RabMAb (catalog #1040-1). |
Product References:
| 1. | Blood, Jun 2006; 107: 4491 - 4499, Requirement of the human T-cell leukemia virus (HTLV-1) tax-stimulated HIAP-1 gene for the survival of transformed lymphocytes ,Katja W'ldele et. al.
[Application: WB] |
Description References:
| 1. |
Rothe, M. et al. The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins. Cell 83(7): 1243 |
| 2. |
Shu, H.B. et al. The tumor necrosis factor receptor 2 signal transducers TRAF2 and c-IAP1 are components of the tumor necrosis factor receptor 1 signaling complex. PNAS USA 93(10): 4974 |
| 3. |
Liston, P. et al. Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes. Nature 379(6563): 349 |
| 4. |
Deveraux, Q.L. et al. IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J. 17: 2215 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
