Clone ID: E35
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1035-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP, FC
Species Reactivity: Human
Mol. Weight: 59 kDa
UniProt ID: Q92851
Alternative names: CASP-10, ICE-like apoptotic protease 4, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2
Description: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-10 (MCH4, FLICE2) is one of the initiator caspases (1,2), and is localized to various tissues. Pro-caspase-10 has two death effector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to form complexes with these receptors and induce apoptosis (1,2). This death domain complex cleaves pro-caspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 leads to the processing of caspase-3 and caspase-7, initiating a caspase cascade and subsequent apoptosis (3,4). It has been shown that the Caspase-10 protein is not produced in neither mouse nor rat (5). Specificity: A synthetic peptide corresponding to N-terminal residues of human Caspase-10 subunit p23/17 was used as immunogen. The antibody does not cross-react with other Caspase family members.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:50-1:100
ICC: 1:100
Flow Cytometry: 1:50
IP: 1:10
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Vincenz, C. and Dixit, V.M. Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem. 272: 6578 |
| 2. |
Fernandes-Alnemri, T. et al. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci., USA 93: 7464 |
| 3. |
Srinivasula, S.M. et al. Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases. Proc. Natl. Acad. Sci., USA 93: 14486 |
| 4. |
Wang, J. et al. Inherited human Caspase 10 mutations underlie defective lymphocyte and dendritic cell apoptosis in autoimmune lymphoproliferative syndrome type II. Cell 98: 47 |
| 5. |
J |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
