Clone ID: E20
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1020-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP, FC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 67 kDa
UniProt ID: O95831
Alternative names: Programmed cell death protein 8
Description: AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4). Specificity: A synthetic peptide correspndonding to residues near the C-terminus of Human AIF was used as immunogen.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:500
ICC: 1:500
Flow Cytometry: 1:50
IP: 1:10
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Product References:
| 1. | Bajt ML et. al., Nuclear Translocation of Endonuclease G and Apoptosis-Inducing Factor during Acetaminophen-Induced Liver Cell Injury ,Toxicol. Sci., Nov (2006) 94: 217 - 225
[Application: IHC] | | 2. | Yu SW et. al, Phagocytosis of apoptotic bodies by hepatic stellate cells induces NADPH oxidase and is associated with liver fibrosis in vivo ,PNAS, Nov 2006; 103:18314 - 18319
[Application: ICC (ms)] |
Description References:
| 1. |
Susin, S.A. et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397(6718): 441 |
| 2. |
Daugas, E. et al. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett. 476(3): 118 |
| 3. |
Daugas, E. et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 14(5):729 |
| 4. |
Susin, S.A. et al. Two distinct pathways leading to nuclear apoptosis. J. Exp. Med. 192(4): 571 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
